Genotipificación de VPH en papilomas orales de pacientes con infección por VIH del Hospital San Juan de Dios / HPV genotypes in oral papilloma in Hospital San Juan de Dios HIV (+) patients

Resumen Introducción: Pacientes con infección por VIH presentan mayor riesgo de infecciones orales con genotipos de virus papiloma humano (VPH) de alto riesgo oncogénico (VPH-AR). Objetivo: Determinar los genotipos de VPH en lesiones papilomatosas orales de pacientes con infección por VIH y describir los factores clínicos, histopatológicos y recuento de linfocitos T CD4+ y carga viral asociados. Métodos: Se estudiaron ocho sujetos adultos con infección por VIH y lesiones papilomatosas por VPH. Se extrajo el ADN de la lesión y se detectó el genoma y los genotipos de VPH mediante reacción de polimerasa en cadena e hibridación con el kit comercial HPV 3.5 LCD-Array (Chipron®) y se describieron factores asociados. Resultados: El 63% de los pacientes exhibió más de un genotipo de VPH y 75% de ellos exhibió al menos un genotipo VPH-AR. El genotipo más frecuente fue el VPH 52 (27%), seguido del VPH16 y 56 (18%). El recuento medio de linfocitos T CD4+ en pacientes con al menos un genotipo VPH-AR fue de 330,6 céls/mm3. Conclusiones: Se detectó una mayor frecuencia de infecciones múltiples por VPH, incluido al menos un genotipo de alto riesgo. El genotipo VPH-AR 52 fue el más frecuente. El recuento medio de LT CD4+ en pacientes que presentan al menos un genotipo VPH-AR indica una inmunosupresión moderada. Se requiere aumentar el número de pacientes.

Background: HIV (+) patients have a higher risk of oral infections with high oncogenic risk HPV (HPV-HR). Aim: To determine the HPV genotypes in oral papillomatous lesions in HIV (+) patients and describe the associated factors. Methods: Eight adults HIV (+) subjects with papillomatous HPV lesions were studied. The lesions DNA was extracted and HPV genome and genotypes were detected by PCR and the commercial kit HPV 3.5 LCD-Array Kit (Chipron®) and associated factors were described. Results: 63% of patients exhibited more than one HPV genotype and 75% of them exhibited at least 1 HPV-HR genotype. The most frequent genotype was HPV 52 (27%), followed by HPV 16 and 56 (18%). The mean CD4 T lymphocyte count in patients with at least one HPV-HR genotype was 330.6 cells/mm3. Conclusions: A higher frequency of multiple HPV infections was detected, including at least one high-risk genotype. The genotype HPV-AR 52 was the most frequent. The mean CD4 T lymphocyte count in patients with at least one HPV-HR genotype indicates moderate immunosuppression. It is required to increase the number of patients.

Prevalence and risk factors for oral human papillomavirus infection in Mexican HIV-infected men / Prevalencia y factores de riesgo para infección oral con virus de papiloma humano en hombres mexicanos con VIH

Abstract: Objective: To determine the prevalence and risk factors for oral high-risk human papillomavirus (HR-HPV) infection in human immunodeficiency virus(HIV)-infected men. Materials and methods: Consecutive male outpatients with HIV-infection were enrolled. Demographic and behavioral risk data were obtained. Anal swabs and oral rinses were tested for HR-HPV DNA. Oral, pharyngeal and video laryngoscopy examinations were performed for detection of lesions. Results: The prevalence of HR-HPV oral infection was 9.3% (subtypes other than HR HPV 16/18 predominated). The prevalence of anal HR-HPV infection was 75.7%. The risk factors for oral infection with HR-HPV were tonsillectomy (OR=13.12) and years from HIV diagnosis (OR=1.17). Conclusions: Tonsillectomy and years from HIV diagnosis were associated with oral HPV infection. No association was found between oral and anal HR-HPV infections. This is the first study reporting the prevalence and risk factors for oral HR-HPV infection in Mexican HIV-infected population.

Resumen: Objetivo: Determinar la prevalencia y los factores de riesgo para infección oral por virus de papiloma humano de alto riesgo (VPH-AR) en individuos con VIH. Material y métodos: Se incluyeron pacientes ambulatorios consecutivos con VIH. Se recabó información demográfica y sobre factores de riesgo conductuales. Se detectó DNA de VPH-AR en hisopado rectal y enjuague bucal. Se efectuó exploración de boca, faringe y videolaringoscopía para detectar lesiones. Resultados: La prevalencia de VPH-AR oral fue 9.3% (predominaron subtipos diferentes de VPH-AR 16/18). La prevalencia de VPH-AR anal fue 75.7%. Los factores de riesgo para VPH-AR oral fueron la tonsilectomía (OR=13.12) y los años de diagnóstico del VIH (OR=1.17). Conclusiones: La tonsilectomía y los años de diagnóstico del VIH se asociaron con VPH-AR oral. No hubo asociación entre VPH-AR oral y anal. Este es el primer reporte sobre prevalencia y factores de riesgo para VPH-AR oral en población mexicana con VIH.

Teat papillomatosis associated with bovine papillomavirus types 6, 7, 9, and 10 in dairy cattle from Brazil

This study describes the clinical, histopathological, and virological characterization of teat papillomatosis from Brazilian dairy cattle herds. Four types of bovine papillomavirus were identified (BPV6, 7, 9, and 10); one of these (BPV7) is being detected for the first time in Brazilian cattle.

Papiloma esofágico: descripción de un caso, identificación molecular del virus del papiloma humano y revisión de la literatura / [Esophageal papilloma: case report, molecular identification of human papillomavirus and literature review].

sophageal squamous papilloma is an uncommon, usually asymptomatic, benign tumor of the squamous epithelium consisting of a raised, sessile, small and round (smooth or rough) lesion. The prevalence is between 0.01 and 0.45

of cases, with a male/female ratio of 3:1. The etiology and pathogenesis appear to be a mechanical or chemical irritation of the mucosa in addition to the presence of human papillomavirus (HPV), important agent in the evolution to a squamous carcinoma, especially HPV types 16 and 18. In this paper, we describe a case of esophageal papilloma whose diagnosis involved endoscopic images, pathological studies and detection of viral DNA by polymerase chain reaction. By using molecular techniques (PCR-RFLP) a profile consistent with HPV type 16 has been obtained. The patient underwent polypectomy and currently, after 3 years of diagnosis, he remains asymptomatic. This work is one of the first national reports of a patient with esophageal papilloma in which one of the most frequently HPV genotypes associated with esophageal carcinoma (HPV 16) has been detected.

Virus del papiloma humano y el cáncer de cabeza y cuello: revisión de la literatura desde México y Colombia / Human papillomavirus and upper aerodigestive tract neoplasm: a review literature from Mexico and Colombia

Antecedentes: Los papilovirus o virus del papiloma son considerados el grupo más prevalentede virus que causa tumores de cabeza y cuello asociados a la infección con virus delpapiloma humano (VPH). Objetivo: Realizar una revisión sistemática de literatura acercadel VPH como agente causal del cáncer de vías aéreas y digestivas superiores (VADS),según información epidemiológica y clínica. Métodos: La búsqueda de literatura biomédicafue efectuada en varias bases de datos, como Medline, Proquest, Science Direct, Ovid yCochrane, así como información disponible en páginas en internet de revistas y organizacionesnacionales. Resultados: Según revisiones sistemáticas sin hallazgos en Colombia,la prevalencia de VPH en el proceso de malignización de lesiones de VADS es 35,6 % enorofaringe, 23,5 % en cavidad oral y 24 % en laringe. En el mundo, la prevalencia de cáncerde cabeza y cuello producido por VPH de alto riesgo, como el VPH 16, es aproximadamentedel 30,9 % en orofaringe, 16 % en cavidad oral y 16,6 % en laringe. Esta revisión destaca losgenotipos de VPH de bajo riesgo (VPH 31, 45, 6 y 11). Se muestra la efectividad del métodoPCR-RFLP como punto de partida para monitorear la infección y su progresión a cáncer,comprender su virulencia y contribuir a la disminución de las tasas de incidencia a causade las imprecisiones de diagnóstico clínico por metodologías convencionales que dificultanla detección precoz del CVADS...

Background: Papillomavirus or papilovirus is considered the most prevalent group of virusesthat cause tumors of head and neck associated with infection with human papillomavirus (HPV). Purpose: Carry out a systematic review of literature on HPV as causal agentof upper aerodigestive tract neoplasm (UADTN), according to epidemiological and clinicaldata. Methods: Pertinent biomedical literature was searched in several databases such asMedline, Proquest, Science Direct, Ovid and Cochrane, as well as available information fromwebsites of national journals and organizations. Results: According to systematic reviewsthat did not show findings in Colombia, the prevalence of HPV in the process of malignantlesions of UADTN is 35.6 %, in the oropharynx, 23.5 %, in oral cavity, and 24 % in larynx.Worldwide, the prevalence of head and neck cancer produced by high-risk HPV, such as HPV16, is approximately 30.9 % in oropharynx, 16 % in oral cavity and 16.6 % in larynx. This reviewalso highlights that the genotypes of low-risk HPV are HPV 31, 45, 6 and 11. It emphasizesthe effectiveness of the PCR-RFLP method as the starting point to monitor the infection andprogression into cancer, to understand its virulence, and to contribute to reduce incidencerates because of the inaccuracies of clinical diagnosis by conventional methods that hinderthe early detection of UADTN...

role of Human papilloma virus [HPV] genotyping in recurrent respiratory papillomatosis in Rasoul Akram Hospital

The most common laryngeal mass in children is recurrent respiratory papillomatosis [RRP]. Studies have attempted to correlate viral typing and its aggressiveness. 29 patients with histologically confirmed RRP enrolled in adjuvant therapies. Patients underwent several surgical interventions. HPV genotyping demonstrated 45% HPV-6 and 55% HPV-11. The mean age at the first surgical intervention was 52.39 months [SD=102.28] [range from 4 months to 426 months]. The mean number of surgical intervention was 10.39 [SD=7.76] [range from 2 to 30]. The mean time of surgical intervals was 4.63 months [SD=4.02] [range from 2 to 24 months]. In fourteen patients [48%] tracheotomy was done. All patients who had tracheotomy received alpha-interferon. One of our cases was a male who had pulmonary extension with HPV-6. A review of patients with RRP was regarding to HPV genotyping and need for adjuvant therapy and tracheostomy. Mean number of surgical procedure was 10/40 and nearly fourteen patients [48%] need to tracheotomy. The clinical differences between HPV6 and HPV11 disease may not be accurately predictable. Patients with less age and with HPV-11 seemed to have more severe problems, but these differences were not statistically significant which needs much more investigations for reasonable starting point of evaluation for these differences

VPH / HPV

Actualmente la Administración de drogas y Alimentos (FdA) de estados unidos aprobó dos vacunas para prevenir la infección por vPh (virus de Papiloma humano): Gardasil® (vacuna tetravalente) y cervarix ® (vacuna bivalente). Ambas vacunas son muy efectivas en la prevención de infecciones persistentes por los tipos 16 y 18 de vPh, dos de los vPh de “alto riesgo” que causan la mayoría (70%) delos cánceres de cuello uterino y en menor porcentaje de cáncer de ano y pene. Gardasil® impide también la infección por los tipos 6 y 11 de vPh, los cuales causan prácticamente todas (90%) las verrugas genitales. se presenta un resumen de la inmunogenicidad, eficacia, indicaciones, modo de empleo y presentaciones comerciales de estas dos vacunas

the Food and drug Administration (FdA) of usA licensed two vaccines for the prevention of vPh (human Papillomavirus) infection: Gardasil® (quadrivalent vaccine) and cervarix® (bivalent vaccine). Both vaccines are very effective in the prevention of persistent infection by serotypes 16 and 18 of vPh, two of the “high risk” vPh, which cause 70% of cervical cancers and in low percentages anal and penile cancers. Gardasil® prevents infection by serotytpes 6 and 11, which cause almost all (90%) of genital warts.this review presents the immunogenicity, efficacy, recommendations, doses, administration and commercial presentation of bothvaccines

Human papillomavirus detection and p16 methylation pattern in a case of esophageal papilloma

Esophageal cancer is a prevalent cancer worldwide. Some studies have reported the possible etiology of human papillomavirus (HPV) in benign and malignant papillomas of the esophagus but the conclusions are controversial. In the present study, we investigated an esophageal papilloma from a 30-year-old male patient presenting aphasia. HPV DNA was detected by generic PCR using MY09/11 primers, and restriction fragment length polymorphism revealed the presence of HPV54, usually associated with benign genital lesions. Hypermethylation of the pINK4A gene was also investigated due to its relation to malignant transformation, but no modification was detected in the host gene. Except for an incipient reflux, no risk factors such as cigarette smoking, alcohol abuse or an infected sexual partner were recorded. Since esophageal lesions may have a malignant potential, HPV detection and typing are useful tools for patient follow-up.

Distribución relativa de genotipos de virus papiloma humano en muestras de carcinoma escamoso de laringe / Relative distribution of human papilloma virus in laryngeal squamous cell carcinoma

Introducción: La relación entre virus papiloma humano (VPH) y cáncer escamoso de la vía aéreo-digestiva superior está claramente establecida en la literatura. Objetivo: El objetivo del presente trabajo es conocer la frecuencia de identificación de ADN de VPH y la distribución relativa de genotipos en muestras de carcinoma escamoso de laringe. Material y método: Se extrajo ADN desde muestras fijadas en formalina e incluidas en parafina, de biopsias de carcinoma escamoso de laringe de pacientes operados en el Servicio de Otorrinolaringología del Hospital San Juan de Dios. La detección de ADN viral se realizó mediante PCR con partidores de consenso MY09/11, y la genotipificación se realizó mediante endonucleasas de restricción Rsal. La calidad de la muestra se controló mediante amplificación de beta-globina. Resultados: Se incluyeron 90 casos. En 24 de ellos (27 por ciento) se identificó la presencia de ADN de VPH. Los genotipos más frecuentes fueron VPH18 (7/24), VPH16 (5/24), VPH54 (2/ 24). En 3 casos no se logró identificar el genotipo. No se detectaron infecciones múltiples. Conclusiones: La presencia de genotipos de VPH de alto riesgo oncogénico sugieren que el virus papiloma humano tendría un rol en la etiopatogenia de un subgrupo de pacientes portadores de carcinoma escamoso de laringe.

Introduction: Human papillomaviruses (HPV) have been detected in benign and neoplastic laryngeal lesions, with variable frequency (20-60 percent). These viral agents are proposed as an adjuvant or cofactor in head and neck carcinogenesis because of their oncogenic properties. Aim: The aim of this study was to identify HPV in laryngeal carcinoma samples and to describe their genotype distribution. Material and method: Tumor samples from patients with newly diagnosed laryngeal carcinomas were collected, fixed in formalin and paraffin-embedded. HPV genome was identified by use of polymerase chain reaction (PCR) using primers complementary to the conserved region L1 (MY09-11). Genotyping was accomplished by restriction fragment length polymorphism. Results: 24 of the 90 samples were positive for HPVDNA (27 percent), all of the samples were positive for human 3-globin. The genotypes identified were HPV 16(5 cases), HPV 18 (7 cases), and HPV 39, 45, 51, 58, 59, 61, and 66 (1 case each). Conclusions: High-risk HPV genotypes were identified, suggesting a role of human papilloma virus in the etiology of a subgroup of laryngeal squamous cell carcinomas.

Aplicação local de cidofovir como tratamento adjuvante na papilomatose laríngea recorrente em crianças / Local application of cidofovir as adjuvant therapy in recurrent laryngeal papillomatosis in children

OBJETIVO: Avaliar a eficácia da aplicação local de cidofovir em associação com o tratamento cirúrgico na papilomatose laríngea recorrente (PLR) em crianças. Desenho do estudo: Prospectivo. MÉTODOS: Quatorze pacientes, com idade média de 4.7 anos e com duas ou mais recidivas após tratamento cirúrgico, foram submetidos à ressecção dos papilomas e injeção de 22.5 mg de cidofovir (7,5 mg/ml) no tecido de onde as lesões foram removidas. Após intervalos de 2-3 semanas, a mesma dose de cidofovir foi repetida duas ou três vezes. Em caso de recidiva, um novo ciclo de cirurgia seguido de aplicações locais de cidofovir era reiniciado. Cinco crianças apresentavam HPV-6 e cinco HPV-11; em quatro casos a tipagem não foi realizada. RESULTADOS: Antes do início do estudo, os pacientes eram submetidos, em média, a duas cirurgias por ano para o controle das recidivas; após o tratamento com cidofovir, a taxa anual de cirurgia diminuiu para 1,1 (p = 0,013). O intervalo médio entre as recidivas antes do início do estudo era de 1.6 meses; ao final do estudo, o intervalo aumentou para 4,4 meses (p = 0,014). Os pacientes com HPV-6 não apresentaram alteração significante nos intervalos entre as recidivas após o tratamento com cidofovir, enquanto 60 por cento das crianças com HPV-11 encontravam-se livres de doença ao final do estudo. CONCLUSÃO: O cidofovir é um adjuvante eficaz no tratamento da PLR em crianças, quando utilizado sob a forma de aplicações locais em associação com a ressecção cirúrgica das lesões. O HPV-11 pode ser mais susceptível aos efeitos benéficos do cidofovir.

OBJECTIVE: Evaluate the efficacy of local application of cidofovir in association with surgical treatment of recurrent laryngeal papillomatosis in children. Study design: Prospective. METHODS: Fourteen patients, with an average age of 4.7 years and with two or more relapses after surgical treatment, were submitted to resection of the papillomas and injection of 22.5 mg of cidofovir (7.5 mg/ml) in the tissue where the lesions had been removed. After 2 to 3 week intervals, the same dose of cidofovir was repeated two or three times. In the case of relapse, a new cycle of surgery followed by local applications of cidofovir was repeated. Five children presented HPV-6 and five HPV-11, while in four, the type was not determined. RESULTS: Before beginning of the study, patients were submitted, on the average, to 2 operations a year for control of relapses. After treatment with cidofovir, the annual rate for surgery dropped to 1.1 (p = 0.013). The average interval between relapses before beginning of the study was 1.4 months; at the end of the study, the interval reached 4.4 months (p = 0.014). Patients with HPV-6 did not show a significant change in the intervals between relapses after treatment with cidofovir, while 60 percent of the children with HPV-11 were disease free at the study end. CONCLUSION: Cidofovir was found to be an effective adjuvant in the treatment of recurrent laryngeal papillomatosis in children, when used in the form of local applications in association with surgical resection of the lesions. HPV-11 may be more susceptible to the beneficial effects of cidofovir.

Papilomatose laríngea: análise morfológica pela microscopia de luz e eletrônica do HPV-6 / Laryngeal papillomatosis: morphological study by light and electron microscopy of the HPV-6

Papilomatose laríngea é neoplasia benigna mais freqüente nas crianças, causada pelo HPV, principalmente subtipos 6 e 11 e caracteriza-se pela presença de lesões proliferativas exofíticas e recidivantes sobre a mucosa das vias aérea, em especial na laringe. Forma de Estudo: Clínico prospectivo. OBJETIVOS: Demonstrar alterações epiteliais morfológicas (pela microscopia de luz e eletrônica) em lesões papilíferas casadas pelo HPV-6. MATERIAL E MÉTODOS: Fragmentos de lesões de papilomatose laríngea, colhidos durante procedimento cirúrgico de quatro crianças (1 masculino, 3 femininas), foram submetidos à tipagem do HPV (por método de PCR), análise pela microscopia de luz e microscopia eletrônica (varredura e transmissão). RESULTADOS: Na tipagem, todos os papilomas eram do subtipo 6. A microscopia de varredura identificou projeções epiteliais de vários tamanhos, com células superficiais em descamação. A microscopia de luz demonstrou lesões exofíticas, revestidas por epitélio hiperplásico com coilócitos e binucleações, característicos do HPV. A membrana basal e o córion adjacente estavam íntegros. À microscopia eletrônica de transmissão identificou-se vacuolização perinuclear e alargamento das junções intercelulares. CONCLUSÕES: As alterações morfológicas apresentadas pelo HPV-6 demonstram o caráter não-invasivo da lesão, sendo necessário estudos morfológicos adicionais relacionando os outros tipos de HPV, considerados mais agressivos, com os achados ultra-estruturais.

Laryngeal papillomatosis is the most frequent benign neoplasia in children. It is caused by HPV 6 and 11. The lesions are exophytic and highly recurrent, compromising the airway mucosa, mainly the larynx. Study design - clinical prospective. AIMS: to show morphologic alterations of the epithelium (light and electron microscopy) in the HPV-6 lesions. METHODS: specimens of laryngeal lesions obtained during surgery of four children (1 male, 3 female) were submitted to HPV typing (PCR), light microscopy and electron microscopy. RESULTS: in all specimens, HPV type 6 was found. Epithelial projections were found by electron microscopy with superficial cells in desquamation. Light microscopy showed exophytic projections of the keratinized stratified squamous epithelium overlying a fibrovascular core. Koilocytes (vacuolated cells), suggesting the viral infection by HPV, were identified. No alterations were seen in the basement membrane and corion. Ultraestrutural analysis showed vacuolated cells with clear cytoplasmic inclusions, intercellular injuries and widening intercellular spaces. CONCLUSIONS: morphologic alterations of the epithelium in the HPV-6 lesions are superficial, and additional studies including the others HPV types are needed to show the more aggressive and extensive aspect of the disease.

Vacuna contra el virus del papiloma humano (VPH) / Vaccine against human papilloma virus (HPV)

El cáncer cuello uterino es el segundo más frecuente en mujeres en el mundo, y constituye una importante causa de mortalidad en nuestro país. La infección con el virus del papiloma humano es reconocida como un co-factor necesario para la génesis de este cáncer. La integración del genoma viral al genoma de la célula huésped altera el ciclo celular normal, favoreciendo su transformación neoplásica. El avance en comprensión de esta relación virus-huésped ha conducido a diseñar una vacuna, cuyo objetivo final sería erradicar esta enfermedad. Este artículo da una perspectiva global del problema y una revisión del conocimiento actual respecto a la vacuna, recientemente implementada en nuestro país.

Relação entre a carga viral de HPV oncogênico determinada pelo método de captura híbrida e o diagnóstico citológico de lesões de alto grau / Association between high-risk human papillomavirus DNA load detected by hybrid capture II and high-grade precursor lesions of cervical cancer in women

INTRODUÇÃO: O papilomavírus humano (HPV) é o principal fator de risco para as neoplasias intra-epiteliais cervicais (NIC) e o câncer cervical. OBJETIVO: O objetivo deste estudo é avaliar se há associação entre a carga viral de HPV oncogênico (alto risco), determinada por meio do teste molecular captura híbrida II (CH II), e o diagnóstico de lesões de alto grau (NIC II/III). MATERIAL E MÉTODOS: Foram analisadas 982 amostras cervicovaginais de exames ginecológicos de rotina, obtidas pelos métodos Papanicolaou convencional e/ou citologia em base líquida (DNA-Citoliq-Digene). Os resultados foram confirmados utilizando-se o método de captura híbrida (CH [Digene]) para detecção de DNA/HPV de alto grau. Os resultados com valor > 1 pg/ml foram considerados positivos, e esses foram divididos em dois grupos: 1. carga viral < 100 pg/ml, e 2. carga viral > 100 pg/ml. RESULTADOS: Dos 210 (21,4 por cento) casos diagnosticados como NIC I, 152 (72,4 por cento) foram positivos para HPV de alto risco por CH II. Desses, 101 (66,4 por cento) apresentaram carga viral > 100 pg/ml. O diagnóstico de NIC II ou III foi confirmado por CH II de alto risco em 86 (43,6 por cento) casos, contudo, entre esses, em 53 (61,6 por cento) a carga viral detectada foi > 100 pg/ml. DISCUSSÃO E CONCLUSÃO: Nossos resultados demonstram que há uma clara associação entre o valor da carga viral determinada pelo método CH II (versão 1) e o grau das lesões precursoras de câncer. Pacientes com carga viral superior a 100 'g/ml devem ser monitoradas periodicamente.

INTRODUCTION: Infection with oncogenic human papilloma virus (HPV) has been established as the main etiologic agent for cervical cancer and of cervical intraepithelial neoplasia (CIN). OBJECTIVE: To determine the association between viral loads of the high risk HPV using the hybrid capture II (HC II) system and CIN lesion stage. MATERIAL AND METHOD: A total of 982 women with diagnosis of negative or of CIN I-III with Pap or liquid-based cytology (DNA-Citoliq-Digene) were included. HC II testing was used to detected HPV DNA. Viral load was measured expressed as relative lights unit (RLU) ratio. RLU ratios were categorized for analysis into three groups: negative (< 1 pg/ml); positive with low viral load (< 100 pg/ml); positive with high viral load (> 100 pg/ml). RESULTS: A total of 152 (72.4 percent) of 210 (21.4 percent) patients with CIN II, 53 (61.6 percent) of 86 (43.6 percent) with CIN II-III were positive for HPV DNA, the higher the viral load (> 100 pg/ml) were observed in 101 (66.4 percent) and 53 (61.6 percent) patients with CIN I or CIN II-III, respectively. DISCUSSION AND CONCLUSIONS: Our results suggest a clear association between viral load HPV DNA determined by HC II (version 1) assay and CIN stage. We concluded that the high viral load has a clinical importance for the management of patients with CIN.

Papiloma escamoso de orelha média: relato de um caso e revisão da literatura

O papiloma escamoso é uma neoplasia benigna. A ocorrência de lesão isolada de orelha média é rara, sendo mais comumente encontrada em associação à patologia nasossinusal. Os autores relatam um caso de papiloma escamoso acometendo apenas orelha média e discutem seus aspectos diagnósticos.

Establishment PCR-based protocol for the detection of human papilloma virus in vaginal swab specimens

The authors reported a PCR-based protocol for the detection of Human papilloma virus in vaginal swab speci

Correlação da infecção viral pelo papilomavírus humano com as lesões papilomatosas e o carcinoma epidermóide na boca e orofaringe / Human papillomavirus (HPV) infection and its relation with squamous cell carcinoma of the mouth and oropharynx

O objetivo deste trabalho é estabelecer a relação entre a infecção pelo vírus do papiloma humano (HPV) e o desenvolvimento de lesões malignas (carcinoma epidermóide) e benignas (hiperplasia epitelial papilomatosa e papiloma) de cavidade bucal e orofaringe. MÉTODOS: Estudo retrospectivo de 66 lâminas e blocos de parafina com tecido biopsiado e fixado com formalina. Esse material, colhido no ano de 2002, refere-se a uma casuística de 44 homens (66,7 por cento) e 22 mulheres (33,3 por cento) com idades que variavam entre 11 e 100 anos. O diagnóstico foi confirmado em 38 casos de carcinoma epidermóide, 20 casos de hiperplasia epitelial papilomatosa e oito casos de papilomas. Utilizamos a imunohistoquímica para analisar este material e determinar a possível presença do DNA viral nas amostras citadas. RESULTADOS: Detectamos a presença do DNA viral (HPV) em 16 dos 38 casos de carcinoma (42,5 por cento), 19 em 20 casos de hiperplasia (95 por cento) e em todos os oito casos de papilomas (100 por cento). CONCLUSAO: Este estudo demonstrou que as lesões papilomatosas e hiperplásicas da boca e orofaringe estão associadas ao HPV, ao contrário do carcinoma espinocelular que não apresentou correlação estatística significante com este agente infeccioso.

Detection of human papillomavirus in epithelial lesions of the conjunctiva

CONTEXT: Many factors like exposure to UV radiation, climatic conditions, genetic predisposition, immunological state and, more recently, the presence of HPV have been implicated in the genesis of some lesions of the conjunctiva, especially the carcinoma. OBJECTIVE: To evaluate the presence of HPV DNA in acquired lesions of the conjunctiva and in normal mucosa. TYPE OF STUDY: Cross-sectional study. SETTING: A public university referral center (the Ophthalmology Service of the A.C. Camargo Hospital - A. Prudente Foundation, Sao Paulo). PARTICIPANTS: Thirty patients with acquired lesions of the conjunctiva and 60 matched controls (by age and sex) were evaluated in this study, from June 1993 to March 1995. PROCEDURES: The detection of HPV DNA in the normal conjunctiva and in acquired lesions was done by the PCR technique and dot blot hybridization. The material was collected by scraping the normal mucosa and the surface of the lesions. A fragment of fresh frozen tissue and paraffin embedded specimens of each lesion were also included. MAIN MEASUREMENTS: The association between the HPV infection and the presence or absence of conjunctival lesions. RESULTS: Sequences of HPV DNA were detected in 4 of the 31 lesions evaluated (12.9 percent) and in the healthy mucosa of one individual of the control group (1.6 percent). HPV type 16 was detected in 2 carcinomas and in the normal mucosa of one individual of the control group. HPV type 11 was demonstrated in 2 papillomas of one patient with lesions in both eyes. CONCLUSIONS: The low frequency of HPV DNA found in the lesions of this sample and the detection of the viral genome in the normal mucosa indicate that there is a weak possibility of association between HPV infection and the carcinoma of the conjunctiva

Infeccao pelo HPV durante o ciclo gravidico-puerperal / Infection caused by HPV during the gravidic puerperal cycle

A infeccao pelo Papiloma Virus Humano (HPV) e patologia que atinge mulheres principalmente durante o periodo reprodutivo. Desse modo, sua associacao com a gestacao e usual e pode implicar em complicacoes tanto para a mae como para o feto. A principal complicacao que atinge o recem-nato e a Papilomatose Recorrente Infantil (PRI). Apesar dos neonatos estarem sob menor risco de exposicao ao HPV com a cesarea, a taxa de infeccao pelo HPV por esta via de parto e especialmente alta, e o parto cesareo pode nao ser eficaz na protecao das...